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$Malvern zetasizer add refractive index$

MALVERN ZETASIZER ADD REFRACTIVE INDEX FULL
MALVERN ZETASIZER ADD REFRACTIVE INDEX SOFTWARE

View the webinars Rational use of dynamic light scattering for the characterization of cytokines, bispecific antibody fusion proteins and binary antibody mixtures and Predicting stability of antibody therapeutics by unfolded-state studies with high-throughput dynamic light scattering (HT-DLS) for more information.Īntibody-drug conjugates (ADCs) are important biotherapeutic candidates that combine highly potent cytotoxic drugs with monoclonal antibodies (mAb) for targeted drug delivery in the treatment of cancer or neurodegenerative disorders. DLS is crucial to understanding the thermal and long-term stability of therapeutically relevant proteins in different formulation conditions. The rational biophysical characterization of therapeutic protein candidates and their formulations can greatly improve the early-stage development process of new biologics.

Integration of plate-based testing protocols with other assays in well-plate format.

Determining the critical aggregation concentration for small molecules such as compound aggregates.

$malvern zetasizer add refractive index$

Assessing multiple formulations for stability, e.g., freeze-thaw cycles, accelerated aging studies or thermal stability screening using the aggregation temperature T agg or melting temperature T m.

Formulation development, e.g., assessment of buffer excipients and pH for colloidal stability using the diffusion interaction parameter k D.

Evaluating the developability of pre-formulations of biotherapeutics.

Quantifying the type and degree of aggregation in hundreds of buffer conditions, important in formulation and crystallization studies.

Some of the high-throughput screening tasks accomplished with the DynaPro Plate Reader include: High-throughput, automated measurements performed directly in standard 96-, 384- or 1536-well microwell plates reveal new horizons for dynamic light scattering. The DLS Theory page contains additional details on the technique, including a video of Brownian motion.

k D and A 2: input sample concentration.

Particle concentration: input sample refractive index.

Temperature is controlled and continuously measured in the instrumentįor advanced measurements, a few more parameters need to be selected or entered into DYNAMICS:.

No sample-specific properties such as concentration or composition are required to assess size, size distributions and aggregation.

MALVERN ZETASIZER ADD REFRACTIVE INDEX SOFTWARE

Moreover, DYNAMICS ® software contains an extensive, built-in solvent library with the most common solvents and buffer systems, including the option to create custom solvents.

Solvent viscosity and refractive index are generally well-known and tabulated.

The only necessary input parameters are solution viscosity and refractive index.ĭLS is a technique that can be readily applied for sizing unknown samples: The resulting intensity fluctuations in the light scattering detector are analyzed to determine a translational diffusion coefficient, which is converted to hydrodynamic radius via the Stokes-Einstein relationship (see sidebar). The sample is illuminated with a laser beam, and light waves scattered from the particles interfere constructively and destructively as the particles diffuse randomly.

$malvern zetasizer add refractive index$

Pre-screening: Ideal to check sample quality before running other expensive and time-consuming biophysical methods such as TEM, AUC, SAXS/SANS, FFF and SEC.įor in-depth characterization of high-resolution size distributions and other physical properties, SEC-MALS and FFF-MALS offer complementary and orthogonal techniques to DLS.ĭLS determines the hydrodynamic size of a nanoparticle in solution by measuring the time scale of Brownian motion.Advanced properties: Measure particle concentration, nonspecific interactions ( A 2 or B 22) and differentiate between conformational changes and aggregation with the static light scattering option in the NanoStar and Plate Reader.Versatility: Beyond size and size distributions, measure other properties such as stability, stress-induced aggregation and molecular interactions.No shearing or filtering: always measure what’s in your solution.Low sample volume: Only 2 µL in the DynaPro NanoStar using quartz cuvettes, 4 µL in disposable cuvettes or standard microwell plates in the DynaPro Plate Reader.Non-destructive and fully recoverable: Samples may be transferred to other assays.

MALVERN ZETASIZER ADD REFRACTIVE INDEX FULL

Full nanometer size range: from 0.5 nm to 2.5 µm in a single measurement, with a resolution up to a factor of 3-5 in size.Dynamic & Electrophoretic Light ScatteringĭLS is an ideal screening tool for biopharmaceutical formulations and other nanomaterials.

$Malvern zetasizer add refractive index$